Select Sample
Solvent:
Petroleum ether : Acetone (9:1)
Chromatography Chamber
baseline
📊 Rf Values
Rf = Distance of spot / Distance of solvent front
Run chromatography to see results
Principle:
Components separate based on differential adsorption and partition between
stationary (paper) and mobile (solvent) phases.
Predict Before You Run
Select a sample above, then predict what the chromatogram will show. The run stays locked until you commit a prediction.
Select a sample to enable prediction.
Lab Journal — Observation vs Interpretation
Select a sample to begin...
⚠️ Safety — Paper Chromatography
- Petroleum ether & acetone are highly flammable: keep well away from any flame, spark or hot plate. No open flames anywhere in the room.
- Volatile, harmful vapours: work in a fume cupboard or well-ventilated area and keep the developing chamber covered so vapour does not escape (this also keeps the atmosphere solvent-saturated for a good separation).
- Skin/eye contact: solvents defat skin and irritate eyes — wear gloves and goggles; avoid inhaling.
- Dyes & extracts stain: handle spotting capillaries carefully and wash hands afterwards. Dispose of solvent as organic waste, not down the sink.